| 摘要: |
| 通过重金属暴露法，研究了不同质量浓度Cd2+（0.005、0.025、0.05、0.1 mg/L）在168 h内对缢蛏消化腺和鳃组织谷胱甘肽硫转移酶（GST）、酸性磷酸酶（ACP）、碱性磷酸酶（AKP）活性的影响。结果显示，Cd2+对两种组织GST活性均有激活作用，且高浓度比低浓度更易快速诱导其活性，但在暴露的后期（>96 h），两个较高浓度组（0.05、0.1 mg/L）GST活性均出现下降；两种组织的ACP活性（鳃组织0.005 mg/L组除外）在暴露的6h即被显著诱导，并表现出剂效关系，但高浓度组更快达到峰值后就持续下降，于暴露中后期（>72 h）被不同程度抑制；消化腺（0.005 mg/L浓度组除外）和鳃AKP活性在暴露168 h内均表现出“抑制-诱导-抑制”的规律，并且较高浓度组活性反而比低浓度组更易被激活，各组AKP活性被诱导达到最高值后就持续下降直至被再次抑制。Cd2+在低浓度水平下即能引起缢蛏两种组织的GST、ACP、AKP活性变化，表明Cd2+的胁迫可对缢蛏机体的解毒体系和新陈代谢产生影响。 |
| 关键词: Cd2+ 缢蛏 谷胱甘肽硫转移酶 磷酸酶 |
| DOI： |
| 分类号: |
| 基金项目:国家科技支撑计划（2009BADB7B02）和浙江省自然科学基金项目（Y3110476） |
|
| Impact of Cd2+ on glutathione S-transferase and phosphatase activity in Sinonovacula constricta |
|
|
| Abstract: |
| Effect of waterborne Cd2+ at different concentrations (0.005mg/L, 0.025mg/L, 0.05mg/L, and 0.1mg/L) on activities of glutathione S-transferase (GST), acid phosphatase(ACP) and alkaline phosphatase (AKP) in digestive glands and gills of Sinonovacula constricta were studied. It was found that the GST activity in the two tissues was stimulated by Cd2+during the exposure time in all treatments, however, those in higher-dose treatments (0.05 mg/L,0.1 mg/L) decreased with the prolonged exposure. The activation of ACP by Cd2+ in the two tissues began at the 6h time-point, and the activity increased with the increasing concentration of Cd2+.For ACP activity, the higher-dose group reached the peak value earlier than the lower-dose group, and was inhibited after 72h. Except 0.005mg/L dose-treatment in digestive glands, the AKP activities in the two tissues showed a“decrease-increase-decrease”trend during the 168h exposure time in the experiment, and those in higher-dose treatments were stimulated earlier than those in lower ones. AKP activity in each treatment decreased constantly after reaching the peak value respectively. The fluctuations in GST, ACP, and AKP activities in the experiment indicated that exposure to lower concentrations of Cd2+ could have affected the metabolism and detoxification system of the organism. |
| Key words: Cd2+ Sinonovacula constricta Glutathione S-transferase Phosphatase |
