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环境因子对圆斑星鲽精子活力的影响
徐永江1, 王妍妍1, 陈学周2, 柳学周1
1.农业部海洋渔业可持续发展重点实验室 青岛市海水鱼类种子工程与生物技术重点实验室 中国水产科学研究院黄海水产研究所,青岛 266071;2.全国水产技术推广总站,北京 100125
摘要:
研究了温度、盐度和pH对圆斑星鲽精子活力的影响。结果表明,圆斑星鲽精子活力最适宜温度范围为8~12℃,最适宜盐度范围为25~35,最适宜pH范围为7~8。由实验结果可知,20℃以上高温和盐度15以下的低盐度海水对圆斑星鲽精子活力有抑制作用,在适宜环境条件下精子快速活动时间最长为7~8min,精子寿命最长为12~17min。生产操作中,圆斑星鲽人工受精应在适宜的环境条件下、精子活力高的时间内进行,以获取高质量的受精卵。
关键词:  圆斑星鲽  精子活力  温度  盐度  pH
DOI:
分类号:
基金项目:国家鲆鲽类产业技术体系建设项目(CARS-50)和国家863计划项目(2012AA10A413)
Influence of environmental factors on sperm activity of Verasper variegatus
Abstract:
The effects of temperature, salinity and pH on the sperm activity of Verasper variegatus were investigated. The results showed that the optimized water temperature for sperm activity ranged from 8 ℃ to 12℃, salinity ranged from 25 to 35, and the most suitable pH range for sperm activity was 7~8. The present results revealed that the activity of sperm was inhibited by high temperature(above 20℃) and low-salinity (<15). Under appropriate environmental conditions the longest eddying time for the spermatozoa was 7~8min and the longest life span was 12~17min. Artificial fertilization should be done under most optimized environmental conditions in order to obtain high quality fertilized eggs. These findings would serve as a reference for the artificial fertilization of V. variegatus.
Key words:  Verasper variegatus  Sperm activity  Temperature  Salinity  pH