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饲料中添加不同水平维生素A对大菱鲆亲鱼繁殖性能的影响
黄利娜1,2, 梁萌青1, 张海涛1, 曲江波3, 王新星1, 郑珂珂1
1.中国水产科学研究院黄海水产研究所, 青岛 266071;2.上海海洋大学水产与生命学院,201306;3.烟台开发区天源水产有限公司,264000
摘要:
以维生素A添加量为0(对照组)、10 000IU/kg(1万组)和80 000IU/kg(8万组)的3种人工配合饲料饲养大菱鲆Scophthamus maximus亲鱼8个月,通过比较各饲料组亲鱼平均相对产卵量、卵子上浮率、受精率、孵化率、卵子与仔稚鱼的大小等,研究不同添加水平的维生素A对亲鱼繁殖性能的影响。结果表明,8万组卵子上浮率和孵化率显著高于对照组(P<0.05),与1万组差异不显著(P>0.05);8万组初孵仔鱼体长2 684.1±24.91μm,显著高于对照组2 562.3±30.00μm(P<0.05),与1万组2 484.2±56.16μm差异不显著(P>0.05);卵子中n-3HUFA、n-6HUFA的含量和性腺中维生素A的含量随着饲料中维生素A添加量的增加而增加;8万组血清中SOD(超氧化物歧化酶,Superoxide dismutase)活力为91.88U/ml,显著高于对照组70.57U/ml(P<0.05)。
关键词:  大菱鲆  亲鱼  维生素A  繁殖性能
DOI:
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基金项目:鲆鲽类产业技术体系科研专项经费(nycytx-50-G07)
The effect of dietary vitamin A level on reproductive performance of broodstock Scophthamus maximus
Abstract:
The eight-month experiment was conducted to determine the effects of vitamin A supplementation (0, 10000, and 80000IU/kg diet) on the growth and reproductive performance of broodstock Scophthamus maximus. Average relative fecundity, percentage of buoyant eggs, fertilization rate, hatching rate of eggs, size of eggs and length of fry were used to evaluate the performance of turbot. The results showed that the percentage of buoyant eggs and hatchability of eggs of the group fed with 80000IU/kg vitamin A were significantly higher than those of the control group(P<0.05). The body length of fry of the group fed with 80000IU/kg vitamin A was 2 684.1±24.91μm, which was significantly higher than those of the control (2 562.3±30.00μm) (P<0.05). The content of n-3HUFA and n-6HUFA in eggs and vitamin A in gonad increased with the increase of vitamin A supplementation levels in diet. SOD activity in serum of broodstock fed with 80000IU/kg vitamin A was 91.88U/ml, which was significantly higher than those of the control (70.57U/ml)(P<0.05). This experiment demonstrated that vitamin A supplemented in the diets could improve the reproductive performance of turbot broodstock.
Key words:  Scophthamus maximus  Broodstock feed  Vitamin A  Reproductive performance