摘要: |
将罗氏沼虾成虾浸浴于含三丁基锡(Tributyltin, TBT) (0.1、0.2、0.4mg/L)的水体中,研究TBT对其肝胰脏和性腺中卵黄蛋白原(VTG)表达和性腺发育的影响。结果表明,罗氏沼虾在TBT中浸浴10d,TBT能够诱导雌虾的肝胰腺VTG基因表达,而对卵巢VTG基因的表达则表现为抑制作用;TBT对雄虾的肝胰腺VTG基因表达有抑制作用,而对精巢VTG基因的表达无明显影响。性腺组织切片显示,TBT浸浴能够促进罗氏沼虾精巢的发育,且随浸浴剂量的增加对精巢发育的促进作用逐步增强,但TBT对罗氏沼虾卵巢发育无明显影响。 |
关键词: 罗氏沼虾 三丁基锡 卵黄蛋白原 性腺 |
DOI: |
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基金项目:广东省自然科学基金(S2011010000254)和广东省科技计划(2008B040006) |
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Effects of tributyltin on vitellogenin expression and gonad development of Macrobrachium rosenbergii |
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Abstract: |
Expression of vitellogenin (VTG) after exposure to tributyltin (TBT) was examined in hepatopancreas and ovarian of Macrobrachium rosenbergii. Results showed that the expression of VTG increased in the hepatopancreas of female M. rosenbergii, but decreased in the ovary. Expression of VTG in the hepatopancreas of male M. rosenbergii was inhibited by TBT, yet no significant effect was observed for TBT on the expression of VTG in the testis. Development of testis in M. rosenbergii was improved after exposure to TBT, but the effect of TBT on ovary development was not significant. |
Key words: Macrobrachium rosenbergii Tributyltin (TBT) Vitellogenin (VTG) Gonad |