| 摘要: |
| 采用富集培养、分离纯化等微生物学手段，从对虾养殖池的生物絮团中筛选出两株对氨氮具有高转化率的菌株。16S rRNA测序及系统发育分析结果表明,两株菌均属于盐单胞菌属，菌株2011072708与食物盐单胞菌Halomonas alimentaria有99%的同源性，而菌株2011072709与胜利盐单胞菌H. shengliensis有100%的同源性。比较研究了两株菌在不同温度、盐度、pH、碳氮比条件下对氨氮的转化率，菌株2011072708在温度37 ℃、盐度30~40、pH 8、碳氮比28的条件下对氨氮的转化率最高；菌株2011072709在温度27~42 ℃、盐度40~50、pH 6、碳氮比21的条件下对氨氮的转化率最高。研究结果表明，胜利盐单胞菌(2011072709)对温度、盐度、pH、碳氮比等各方面的适应性优于食物盐单胞菌(2011072708)，更适合在生物絮团技术中得到应用。 |
| 关键词: 生物絮团 异养亚硝化细菌 氨氮转化率 盐单胞菌 |
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| 基金项目:公益性行业（农业）科研专项经费项目(201103034)和现代农业产业技术体系(CARS-47) |
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| Isolation and identification of heterotrophic nitrosobacteria from bio-flocs and analysis of their characteristics |
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| Abstract: |
| Two strains of nitrosobacteria with high ammonia transformation rate were isolated by enrichment and selective culture from bio-flocs of shrimp culture ponds. Sequences of 16S rRNA indicated that both strains belonged to the genus Halomonas sp., while strain 2011072708 was identified as H. alimentaria with 99% similarity, and strain 2011072709 as H. shengliensis with 100% similarity. Ammonia transformation rates of the two strains were compared under different temperatures, salinities, pH, and C/N ratios. The ammonia transformation rate of strain 2011072708 reached the highest level at 37 ℃, 30~40, pH 8, and C/N ratio of 28, while that of strain 2011072709 reached the maximum at 27~42 ℃, 40~50, pH 6, and C/N ratio of 21. The present study showed high ammonia transformation rates in both nitrosobacteria strains isolated from bio-flocs. Strain H. shenliensis (2011072709) had better adaptability to temperature, salinity, pH, and C/N ratio than H. alimentaria (2011072708), and may be more suitable for application in bio-flocs technology. |
| Key words: Bio-flocs Nitrosobacteria Ammonia transforming rate Halomonas sp. |
