| 摘要: |
| 运用亚细胞分级方法分析羊栖菜亚细胞组分中6种必需元素Cu、Fe、Mn、Zn、Mg、Ca以及有害元素Cd、Pb、Al、As的含量，以期揭示羊栖菜中不同微量元素的亚细胞分区分布特征，探讨其特异性富集砷的特性。研究结果表明，Cu、Fe、Mn、Zn、Ca、Mg等必需微量元素主要储存在细胞壁中，而在胞液和细胞器中的分布特征不同。对于Al，Cd和Pb，细胞壁中的浓度远高于细胞器和胞液，但是对于As，胞液是最主要的储存部位，分布比例约为58%，其次为细胞壁（约为26%～27%），最后为细胞器（约为15%～16%）。通过本实验推断胞液对As的区隔化作用很可能是羊栖菜特异性富集As和解毒As的重要原因。 |
| 关键词: 羊栖菜 特异性富集 微量元素 亚细胞分级 |
| DOI：10.11758/yykxjz.20130618 |
| 分类号: |
| 基金项目:中央级科研院所基本科研业务费（20603022012016） |
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| Subcellular distributions of trace metals in Sargassum fusiforme |
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| Abstract: |
| The subcellular distributions of Cu, Fe, Mn, Zn, Mg, Ca, Cd, Pb, Al, and As in Sargassum fusiforme were examined, in order to reveal the subcellular distribution characteristics of different elements and the mechanism of its special hyperaccumulation of As. The results indicated that Cu, Fe, Mn, Zn, Ca and Mg were mainly stored in the plant cell walls, yet their distributional patterns in cytoplasmic supernatant and cell organelles were different. The concentrations of Al, Cd and Pb in the cell walls were much higher than those in either cytoplasmic supernatant or cell organelles. However, the subcellular distributions of As showed a decreasing trend as: cytoplasmic supernatant (58%) >cell wall (26%-27%)>cell organelles (15%-16%), indicating that the cytoplasmic supernatant was the main storage for As in S. fusiforme. The present study suggested that the isolation of As in cytoplasmic supernatant may be an important mechanism for accumulation of As in S. fusiforme. |
| Key words: Sargassum fusiforme Special hyperaccumulation Trace metals Subcellular distributions |
