| 摘要: |
| 本研究采用2×5双因子试验设计，即在基础饲料中分别添加0和0.15 mg/kg的Se，每一Se水平下分别添加0、30、60、90、120 mg/kg的维生素E(VE)，共制成10种试验饲料，饲喂平均初始体质量为(0.37±0.01) g的吉富罗非鱼(Oreochromis niloticus) 70 d，每组设3个重复，每个重复组50尾吉富罗非鱼，其中饲喂未添加Se和VE的基础饲料组为对照组。结果表明，(1) VE对吉富罗非鱼幼鱼增重率、特定生长率、摄食量和饲料系数均有显著影响(P<0.05)，就生长性能而言，单独添加VE的适宜范围是63.86–70.58 mg/kg。Se对吉富罗非鱼的增重率、特定生长率的影响不显著(P>0.05)，但在VE 添加量为0 mg/kg的试验组中，硒的添加使吉富罗非鱼增重率高于对照组。VE和Se对吉富罗非鱼生长的交互作用没有显著影响(P>0.05)。(2) VE对谷胱甘肽过氧化物酶(GSH-PX)和过氧化氢酶(CAT)活性有显著影响(P<0.05)，总超氧化物歧化酶(T-SOD)和CAT活性随VE添加量的增加呈上升趋势。而Se可显著影响T-SOD和GSH-PX的活性(P<0.05)，在VE添加量范围60–90 mg/kg时，加Se试验组的T-SOD、GSH-PX和CAT活性较不加Se组有所上升。VE和Se对GSH-PX活性的影响具有显著的交互作用(P<0.05)。在本研究条件下，VE和Se对吉富罗非鱼的生长及抗氧化有一定促进作用，二者联合使用，没有协同促生长作用，但抗氧化作用得到进一步加强。 |
| 关键词: 吉富罗非鱼 维生素E 硒 生长 抗氧化酶活性 |
| DOI：10.11758/yykxjz.20140412 |
| 分类号: |
| 基金项目:国家科技支撑计划(2012BAD25B04)、广西重大科技专项(14121004-2-2)、广西科技攻关项目(0992014-2)、广西南宁市科技攻关项目(201102100G)共同资助 |
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| Effects of Dietary Vitamin E and Selenium on the Growth and the Antioxidant Enzyme Activity in Serum of Juvenile Tilapia (Oreochromis niloticus) |
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| Abstract: |
| In this study we investigated the effects of dietary vitamin E and selenium on the growth and the antioxidant enzyme activities in serum of juvenile tilapia (Oreochromis niloticus). A total of 1500 tilapias with an average initial body weight of (0.37±0.01) g were fed for 70 days with 10 different practical diets according to a 2×5 factorial design: selenium was added into a basal diet at two concentrations (0 and 0.15 mg/kg) to form two types of diets; in each type vitamin E was added at 0, 30, 60, 90 or 120 mg/kg separately to form 5 groups; there were 10 groups in total; there were 3 replicates per group with 50 tilapias; the basal diet without selenium or vitamin E served as the control. The results showed that dietary vitamin E had significant influence on the weight gain, the specific growth rate, feed intake, and the feed conversion ratio (P<0.05). According to the growth performance, the optimal level of vitamin E in the diet could be 63.8670.58 mg/kg. Dietary selenium had no significant influence on the weight gain and the specific growth rate (P>0.05), but the weight gain was higher than the control group when the diet was supplemented with 0.15 mg/kg selenium and 0 mg/kg vitamin E. Dietary vitamin E and selenium had no significant influence on the growth performance (P>0.05). Dietary vitamin E significantly influenced the activities of glutathione peroxidase (GSH-PX) and catalase (CAT) (P<0.05). The activities of total superoxide dismutase (T-SOD) and CAT also appeared to increase along with the elevation of the vitamin E level. Selenium had significant influence on the activities of GSH-PX and T-SOD (P<0.05). When the added vitamin E was between 60 and 90 mg/kg, the groups with 0.15 mg/kg selenium show an increase in the activities of T-SOD, GSH-PX and CAT compared to the groups without selenium. Dietary vitamin E and selenium had significant interactive effect on the activity of GSH-PX (P<0.05). In conclusion vitamin E or selenium alone could improve the growth performance and the anti-oxidation activity in tilapias; the combination of the two may not further enhance the growth performance but could increase the anti-oxidation activity to a greater extent. |
| Key words: Oreochromis niloticus Vitamin E Selenium Growth Antioxidant enzyme activity |
