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| 贝类毒素固相吸附示踪监测技术研究进展 |
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李兆新1,2, 张婷婷1,3, 郭萌萌1,2, 孙晓杰1,2, 宋才湖1,3, 周德山4, 宋新成4
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1.中国水产科学研究院黄海水产研究所 青岛 266071;2.农业部水产品质量安全检测与评价重点实验室 青岛 266071;3.上海海洋大学食品学院 上海 201306;4.连云港市水产品质量检测中心 连云港 222001
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| 摘要: |
| 固相吸附毒素示踪监测技术(Solid Phase Adsorption Toxin Tracking，SPATT)是一项新的贝类毒素监测技术。该技术模拟滤食性贝类摄食过程，利用内含吸附剂的采样器富集水体中的贝类毒素，结合高效液相色谱-质谱检测技术或酶联免疫检测技术，可以对产毒藻类的暴发以及贝类毒素污染进行示踪研究。SPATT监测技术与贝类监测法、浮游藻类监测法相比，具有取样操作简单、检测成本低等优点，已成为贝类毒素污染监测技术研究的热点。本综述针对SPATT技术的研究进展及应用进行阐述，对技术适用监测的毒素种类、吸附材料的优劣等进行分析。 |
| 关键词: 固相吸附毒素示踪监测技术 贝类毒素 吸附剂 |
| DOI：10.11758/yykxjz.20150703002 |
| 分类号: |
| 基金项目:农业部引进国际先进农业科学技术计划项目(NO.948-2013-S8)资助 |
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| Review of the Development and Application of Solid Phase Adsorption Toxin Tracking for Shellfish Toxin Monitoring |
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LI Zhaoxin1,2, ZHANG Tingting1,3, GUO Mengmeng1,2, SUN Xiaojie1,2, SONG Caihu1,3, ZHOU Deshan4, SONG Xincheng4
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1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;2.Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Qingdao 266071;3.College of Food Science and Technology, Shanghai Ocean University,Shanghai 201306;4.Lianyungang Center for Quality Testing of Aquatic Products, Lianyungang 222001
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| Abstract: |
| Toxins produced by harmful algae has been a global food safety issue in aquaculture industry. Unlike conventional phytoplankton and shellfish monitoring techniques, solid phase adsorption toxin tracking (SPATT) simulates the contamination of filter feeding bivalves and employed passive sampling of adsorption substrates. Combined with appropriate assays such as HPLC-MS/MS and ELISA that purify and analyze algal toxins, SPATT gives early warning of harmful algal blooms and the subsequent contamination of shellfish. In this review we discussed the progress and prognosis on studies of SPATT. We elaborated the advantage of SPATT in detecting marine toxins including diarrhetic shellfish poisoning, paralytic shellfish poisoning, amnesic shellfish poisoning, ciguatoxins, cyanotoxins, and homoanatoxins. We also summarized the function of different absorbent resins such as HP-20, SP-70, SP700, SP850, SP825L, XAD4, and L-493. SPATT is an inexpensive and convenient tool for fast detection of algal toxins. |
| Key words: Solid phase adsorption toxin tracking (SPATT) Shellfish toxin Adsorbent |