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| 中国对虾NAGase基因SNP标记的筛选及与耐高pH性状的关联分析 |
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韩旭1,2, 何玉英3,2, 李健3,2, 张海恩2, 周雨欣1,2
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1.上海海洋大学水产科学国家级实验教学示范中心 上海 201306;2.中国水产科学研究院黄海水产研究所 农业农村部海水养殖病害防治重点实验室 青岛 266071;3.青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071
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| 摘要: |
| 为探究N-乙酰-β-D-氨基葡萄糖苷酶基因(NAGase)对中国对虾(Fenneropenaeus chinensis)耐高pH性状的影响，本研究采用直接测序法及实时荧光定量PCR法检测FcNAGase的多态性，并分析其与中国对虾耐高pH性状的相关性。通过直接测序，在FcNAGase上共检测到29个潜在的SNP位点，分布频率为1.13/100 bp，其中，内含子的突变频率为0.35/100 bp，外显子的突变发生频率为0.78/100 bp。通过荧光定量PCR方法在中国对虾耐高pH性状分离群体中共检测到14个具有多态性的SNP位点，其中，观测杂合度(Ho)为0.2581~0.9390，期望杂合度(He)为0.0595~0.4490，多态信息含量(PIC)为0.0574~0.3731；利用卡方检验进行关联性分析，发现2个与中国对虾耐高pH性状显著相关的位点(P<0.05)，分别为P12 (P=0.036)和P27 (P=0.018)，本研究结果可为中国对虾分子标记辅助育种研究提供基础数据和方法。 |
| 关键词: 中国对虾 NAGase基因 耐高pH性状 SNP位点 |
| DOI：10.19663/j.issn2095-9869.20191205003 |
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| Correlation between the SNP of NAGase gene and susceptibility to high pH stress in Fenneropenaeus chinensis |
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HAN Xu1,2, HE Yuying3,2, LI Jian3,2, ZHANG Haien2, ZHOU Yuxin1,2
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1.National Experimental Teaching Demonstration Center of Aquatic Science, Shanghai Ocean University, Shanghai 201306;2.Key Laboratory for Disease Control of Mariculture, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;3.Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071
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| Abstract: |
| To explore the effect of the beta-N-acetylglucosaminidase (NAGase) gene on the high pH tolerance of Fenneropenaeus chinensis, the single nucleotide polymorphism (SNP) of FcNAGase and its correlation with the high pH tolerance of F. chinensis were determined by direct sequencing and real-time fluorescent quantitative PCR. The results showed that 29 potential SNP loci were detected on the FcNAGase gene by direct sequencing, the distribution frequency was 1.13/100 bp, in which the mutation frequency of introns was 0.35/100 bp, and that of exons was 0.78/100 bp. Fourteen SNP loci were detected in the high pH tolerance population of F. chinensis using fluorescence quantitative PCR. The observed heterozygosity (Ho) ranged from 0.2581 to 0.9390, the expected heterozygosity (He) ranged from 0.0595 to 0.4490, and the polymorphism information content (PIC) ranged from 0.0574 to 0.3731. The chi-square test result revealed that two SNP loci were related to high pH tolerance traits (P<0.05), namely P12 (P=0.036) and P27 (P=0.018). The results of this experiment provided basic data and methods for molecular marker assisted breeding of F. chinensis. |
| Key words: Fenneropenaeus chinensis NAGase gene High pH resistance SNP site |