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| 刺参“参优1号”新品种在不同盐度下的代谢特征和适应性研究 |
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王治平1,2, 李彬3,4, 秦蕾1, 王印庚1,3,4, 廖梅杰3,4, 荣小军3,4, 张正3,4, 范瑞用5, 郑炯6
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1.江苏海洋大学 连云港 222005;2.中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 青岛 266071;3.中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展
重点实验室 青岛 266071;4.青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071;5.青岛瑞滋集团有限公司 青岛 266408;6.青岛市渔业技术推广站 青岛 266000
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| 摘要: |
| 本研究通过测定盐度为14~40条件下，刺参“参优1号”苗种的生长、存活、呼吸代谢及非特异性免疫酶活性差异，确定其适宜和最适盐度条件及其盐度适应机制。结果显示，盐度为23~40时，30 d苗种的存活率(SR)均为100%；盐度在16以下时，苗种全部死亡。盐度为23~40时，苗种的特定生长率(SGR)为正值；盐度为29~37时，SGR较高，为0.9932%/d，盐度为20以下时，SGR降为负值。耗氧率(RO)和排氨率(RN)随盐度的变化呈现出以32为波谷“M”型变化，盐度为32条件下的RO和RN分别为0.0130和0.00138 mg/(g·h)。不同盐度组刺参的O : N值均为8左右，O : N值随盐度升高无显著差异。盐度的升高和降低会引起刺参体腔液中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、溶菌酶(LZM)、超氧化物歧化酶(SOD)活性显著升高(P<0.05)，在苗种适应盐度胁迫过程中，各盐度组SOD活性的峰值一般在0 d出现，而ACP、AKP活性在第10天出现峰值，LZM活性的峰值在10~20 d。研究表明，刺参“参优1号”适宜的生长盐度为23~40，最适盐度范围为29~37，盐度变化会导致苗种呼吸代谢和免疫酶活性的变化，研究结果将为刺参“参优1号”苗种的推广提供科学依据。 |
| 关键词: 刺参“参优1号” 盐度适应 生长 呼吸代谢 非特异性免疫酶活性 |
| DOI：10.19663/j.issn2095-9869.20200331002 |
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| Metabolic characteristics and adaptability of a new variety of sea cucumber “Shenyou No.1” under different salinities |
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WANG Zhiping,LI Bin,QIN Lei,WANG Yingeng,LIAO Meijie,RONG Xiaojun,ZHANG Zheng,FAN Ruiyong,ZHENG Jiong
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1.Jiangsu Ocean University, Lianyungang 222005;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071;3.Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071;4.Qingdao Ruizi Group Co., Ltd, Qingdao 266408;5.Qingdao Fishery Technical Extension Station, Qingdao 266000
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| Abstract: |
| Salinity is a key ecological factor for the survival of the sea cucumber (Apostichopus japonicus), and analysis of the adaptation and tolerance mechanisms of new sea cucumber varieties can provide a scientific basis for their promotion. To study the adaptation of a new variety of sea cucumber, “Shenyou No. 1,” the growth, survival, respiratory metabolism, and immune enzyme activities in different salinities (ranging from 14 to 40) were measured. The results revealed that the survival rate was 100% within the salinity range of 23~40, whereas all seedlings died when the salinity was below 16. The specific growth rate (SGR) was positive within the salinity range of 23~40 and reached a peak within the salinity range of 29~37, with the highest SGR being 0.9932%/d at a salinity of 32. With a change in salinity, the oxygen consumption rate and ammonia excretion rate showed a “M” type change, with the trough values obtained at a salinity of 32 with values of 0.0130 mg/(g·h) and 0.00138 mg/(g·h), respectively. The O/N ratio showed no significant difference with the change in salinity. The ACP, AKP, LZM, and SOD activities changed significantly under the high and low salinity stresses. During the adaptation period, the peak of SOD enzyme appeared at 0 d, whereas the peak of ACP and AKP appeared at 10 d, and the peak of LZM appeared at 10~20 d. All the results indicated that the suitable salinity range for the new variety “Shenyou No. 1” was 23~40 and the optimum salinity range was 29~37. Changes in the salinity led to changes in the respiratory metabolism and immune enzyme activity of the seedlings. These results provide a scientific basis for the promotion of the new variety of sea cucumber “Shenyou No. 1.” |
| Key words: Sea cucumber “Shenyou No.1” Salinity adaptation Growth Respiratory metabolism Immune enzyme activities |