引用本文:
【打印本页】   【下载PDF全文】   View/Add Comment  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 611次   下载 538 本文二维码信息
码上扫一扫!
分享到: 微信 更多
酵母水解物对吉富罗非鱼生长、免疫力、抗氧化性及抗无乳链球菌感染的影响
董兰芳,张彩群,刘家辉,郑晓利,童潼,张琴,王秀华,杨慧赞
1.广西民族大学海洋与生物技术学院 广西多糖材料与改性重点实验室 广西高校微生物与植物资源利用重点实验室 广西 南宁 530006;2.广西海洋研究所有限责任公司 广西 北海 536000;3.中国水产科学研究院黄海水产研究所 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 农业农村部海水养殖病害防治重点实验室 青岛市海水养殖流行病学与生物安保重点实验室 山东 青岛 266071;4.广西水产科学研究院 广西水产遗传育种与健康养殖重点实验室 广西 南宁 530021
摘要:
本研究旨在探究饲料中添加酵母水解物对吉富罗非鱼(Oreochromis niloticus)生长性能、免疫力和抗病力的影响。在基础饲料中添加0 (对照组)、0.5%、1.0%、1.5%和2.0%的酵母水解物制成5种实验饲料,对5组初始体重为(25.80±0.45) g的吉富罗非鱼进行为期8周的养殖实验,每组 3个重复,每个重复30尾鱼。实验结束后,每个重复选择12尾鱼进行无乳链球菌(Streptococcus agalactiae)攻毒实验,每尾鱼腹腔注射0.2 mL浓度为8.3×105 CFU/mL的菌液,连续观察14 d,统计累积死亡率。结果显示,随着酵母水解物添加量的增加,吉富罗非鱼终末体质量(FBW)和特定生长率(SGR)均呈先显著上升(P<0.05)后下降的趋势。回归分析表明,当SGR达到最大值时,对应的酵母水解物添加量为1.29%。罗非鱼血清一氧化氮合酶、酚氧化酶、酸性磷酸酶、过氧化氢酶和超氧化物歧化酶活性均随饲料中酵母水解物添加量的提高呈先上升后下降的趋势(P<0.05),添加量为1.5%时达最大值。饲料添加酵母水解物对吉富罗非鱼无乳链球菌的抵抗力有显著影响(P<0.05),除添加量为0.5%的实验组死亡率与对照组差异不显著外,其他实验组罗非鱼的死亡率均显著低于对照组(P<0.05)。本研究中,饲料添加酵母水解物可有效提高吉富罗非鱼的生长性能、免疫力和抗病力,且在添加量为1.5%时,效果最显著。
关键词:  吉富罗非鱼  酵母水解物  生长  免疫酶  抗氧化酶  无乳链球菌
DOI:
分类号:
基金项目:
Effects of Yeast Hydrolysate on Growth, Immunity, Oxidation Resistance, and Anti-Streptococcus agalactiae Activity of Genetically Improved Farmed Tilapia
DONG Lanfang1,2, ZHANG Caiqun1, LIU Jiahui1, ZHENG Xiaoli1, TONG Tong1, ZHANG Qin1,2, WANG Xiuhua3,4,5,6, YANG Huizan7
1.School of Marine Science and Biotechnology, Guangxi University for Nationalities, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, Nanning, Guangxi 530006, China;2.Guangxi Ocean Research Institute Co., Ltd, Beihai, Guangxi 536000, China;3.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;4.Laboratory of Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao);5.Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs;6.Key Laboratory of Maricultural Epidemiology and Biosecurity of Qingdao, Qingdao, Shandong 266071, China;7.Guangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, Guangxi 530021, China
Abstract:
This study aimed to investigate the effects of yeast hydrolysate on the growth performance, immunity, oxidation resistance, and anti-Streptococcus agalactiae activity of genetically improved farmed tilapia (GIFT). Five experimental diets were prepared by adding 0 (control group), 0.5%, 1.0%, 1.5%, and 2.0% yeast hydrolysate to the basic diet of these fish. GIFT with an initial weight of (25.80±0.45) g were randomly divided into five groups and were cultured for 8 weeks. After the breeding test, 12 fish from each group were selected for the S. agalactiae challenge test. Each fish was injected intraperitoneally with 0.2 mL bacterial solution at a concentration of 8.3×105 CFU/mL, and the cumulative mortality was counted for 14 days. The results showed that the final body weight and specific growth rate (SGR) increased significantly at first (P<0.05) and then decreased with the increase in the concentration of yeast hydrolysate. Regression model analysis showed that the most suitable dietary yeast hydrolysate level reached the highest SGR at 1.29%. The activities of nitric oxide synthase, phenol oxidase, acid phosphatase, catalase, and superoxide dismutase increased (P<0.05) at first and then decreased under the increased dietary yeast hydrolysate level; the peak was reached on supplementation with 1.5% yeast hydrolysate. Dietary yeast hydrolysate significantly influenced the resistance of GIFT to S. agalactiae (P<0.05). Except for the 0.5% yeast hydrolysate test group, the cumulative mortalities of the other test groups (1.0% yeast hydrolysate supplementation or more) were significantly lower than the cumulative mortality of the control group (P<0.05). In this study, the addition of yeast hydrolysates to the diet of GIFT significantly improved their growth performance, immunity, and disease resistance, and the 1.5% yeast hydrolysate-supplemented test group showed the best results.
Key words:  Oreochromis niloticus (GIFT)  Yeast hydrolysate  Growth  Immune enzyme  Antioxidase  Streptococcus agalactiae