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共培养系统中4种微藻生态因子的研究
文国樑1, 梁伟峰1, 李卓佳2, 曹煜成1, 陈素文1
1.中国水产科学研究院南海水产研究所,广州 510300);2.广东海洋大学,湛江 524000)
摘要:
通过二次回归通用旋转组合设计安排实验,研究光强、温度和盐度对分离自对虾池塘水环境中的啮蚀隐藻、新月菱形藻、微绿球藻和蛋白核小球藻的增长率的影响。获得各微藻的最适生态因子及其影响度。啮蚀隐藻的最适生态因子为:光强5 750~7 944 lx,温度21.3~28.3 ℃,盐度13.3~23.0;影响度依次为:盐度>温度>光强。新月菱形藻的最适生态因子为:光强5 761~8 697 lx,温度23.4~29.6 ℃,盐度11.9~25.7;影响度依次为:盐度>光强>温度。蛋白核小球藻的最适生态因子为:光强6 754~8 775 lx,温度17.1~20.7 ℃,盐度19.6~26.4;影响度依次为:温度>盐度>光强。微绿球藻的最适生态因子为:光强7 128~9 012 lx,温度18.7~26.7 ℃,盐度17.9~24.3;影响度为光强>温度>盐度。啮蚀隐藻和新月菱形藻的增长率受到以盐度为基础、以光强和温度为协同因子的显著影响。
关键词:  啮蚀隐藻  新月菱形藻  蛋白核小球藻  微绿球藻  光强  温度  盐度
DOI:
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基金项目:国家十一五支撑计划(2006BAD09A11、2006BAD09A07)、南海水产研究所中央级科研院所基本科研专项(2007ZD01)、农业部行业专项(nyhyzx07 042)、广东省科技计划(2006B20601009和2008A200100002)和现代农业(虾)产业技术体系建设专项资金共同资助
Studies on the ecological factors of four microalgae populations in mixed-culture
Abstract:
By means of the common rotation composite design of quadratic regression, four mathematical models were established to analyze the impact of light intensity (x1), temperature (x2) and salinity (x3) on cell division rate (k) of each microalgal species seperated from shrimp ponds in mixed culture. The suitable conditions for the microalgae growth and the weightiness of each factor to the cell division rate were obtained. The suitable light intensity for Cryptomonas erosa ranged from 5 750 lx to 7 944 lx, the suitable temperatures ranged from 21.3 ℃ to 28.3 ℃ and the suitable salinity ranged from 13.3 to 23.0. The weightiness of the impact of each factor on cell division rate was x3>x2>x1. The suitable light intensity for Nitzschia closterium ranged from 5 761 lx to 8 697 lx, the suitable temperatures ranged from 23.4 ℃ to 29.6 ℃ and the suitable salinity ranged from 11.9 to 25.7. The weightiness of the impact of each factor on cell division rate was x3>x1>x2. The suitable light intensity for Nannochloropsis oculata ranged from 6754lx to 8775lx, the suitable temperatures ranged from 17.1 ℃ to 20.7 ℃ and the suitable salinity ranged from 19.6 to 26.4. The weightiness of the impact of each factor on cell division rate was x2>x3>x1.The suitable light intensity conditions for Chlorella pyrenoidosa ranged from 7 128 lx to 9 012 lx, the suitable temperatures ranged from 18.7 ℃ to 26.7 ℃ and the suitable salinity ranged from 17.9 to 24.3. The weightiness of the impact of each factor on cell division rate was x1>x2>x3. The k of Cryptomonas erosa and Nitzschia closterium was significantly affected by salinity acting synergistically with light intensity and temperature.
Key words:  Cryptomonas erosa  Nitzschia closterium  Nannochloropsis oculata  Chlorella pyrenoidosa  Light intensity  Temperature  Salinity