| 摘要: |
| 选择三疣梭子蟹22个多态性高的微卫星标记，经过引物间的搭配、组合测试以及反应条件的优化（退火温度、Mg2 +含量、dNTPs的浓度等参数），构建了多重PCR扩增技术体系。本研究最终建立了47个二重PCR组合，并在47个二重引物的组合基础上建立15个三重PCR组合，3个四重PCR组合。该多重PCR技术体系的建立为三疣梭子蟹品种选育、种系评估提供快速、有效分子检测技术。 |
| 关键词: 三疣梭子蟹 SSR 多重PCR体系 |
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| 基金项目:国家高技术研究发展计划项目（2006AA10A406）、国家自然科学基金（30871933）、农业科技成果转化资金项目（2010GB23260589）共同资助 |
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| Establishment and optimization of a microsatellite-labeling multiplex PCR system in Portunus trituberculatus |
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| Abstract: |
| By selecting 22 pairs of polymorphic microsatellite markers of Portunus trituberculatus, primers matching, and combinatorial testing, the multiplex PCR amplification system was built and the reaction conditions were optimized, including annealing temperature, Mg2+ concentration, dNTPs concentration and other parameters. Forty-seven combinations of double PCR, 15 combinations of triple PCR, and 3 quadruple PCR combinations were established. The establishment of this technology will provide a fast, effective molecular detecting tool for the P. trituberculatus breeding and germ-line assessment. |
| Key words: Portunus trituberculatus SSR Multiplex PCR system |
