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条纹锯鮨胚胎发育及卵黄囊仔鱼形态变化的观察
贾瑞锦1,2, 王鲁2, 赵从明3, 公光业1,2, 孙曙光2, 于宏2, 王彦怀3, 孙涛4, 王贺3, 陈超2
1.上海海洋大学水产与生命学院,201306;2.农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所,青岛 266071;3.天津立达海水资源开发有限公司,300050;4.烟台开发区天源水产有限公司,264000
摘要:
通过观察条纹锯鮨的胚胎发育过程及卵黄囊仔鱼形态变化,研究其胚胎发育及卵黄囊仔鱼的形态特征、特点。采用Nikon E200显微镜对其胚胎发育形态及卵黄囊仔鱼形态变化进行连续拍照取样,详细描述了其从受精卵到初孵仔鱼的26个具体发育时期的形态特征、孵化温度、发育时间及卵黄囊仔鱼的形态变化特征。结果显示,条纹锯鮨的受精卵为球形、游离的浮性卵,平均卵径为0.950±0.039mm;条纹锯鮨受精卵孵化需经过卵裂期、囊胚期、原肠胚期、神经胚期、器官生成期和孵化期6个主要时期,在80目的孵化网箱中,水温20~20.5℃、盐度28~32、DO≥5mg/L、pH 7.6~8.4静水微充气的条件下需要46 h 53 min孵化;卵黄囊在仔鱼出膜2 d后被吸收。
关键词:  条纹锯鮨  胚胎发育  卵黄囊仔鱼  受精卵
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基金项目:科技部国际合作专项(2012DFA30360)和天津市滨海新区项目(201004070)
Preliminary studies on embryonic development and morphology of the yolk sac larvae of Centropristis striata Linnaeus
Abstract:
Nikon E200 microscope was used to observe the embryonic development of Centropristis striata Linnaeus. Detailed changes from the fertilized egg to the newly hatched fish, and morphological characteristics of 26 specific developmental stages were described. The results showed that fertilized eggs of C. striata Linnaeus were free-floating and spherical, at an average diameter of 0.950±0.039mm; Embryonic development C. striata Linnaeus experienced six main stages including cleavage, blastocyst, gastrulation, neurula embryo, organogenesis and hatching stage. The results showed that, at water temperatures of 20~20.5℃,salinity 28~32, DO≥5mg/L, and pH 7.6~8.4,fertilized eggs of C. striata maintained in cages with slight areation were hatched in 47 h, and yolk-sac in the larvae disappeared after 2 days.
Key words:  Centropristis striata Linnaeus  Embryonic development  Yolk sac larvae  Fertilized egg