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| 半滑舌鳎生长相关基因的微卫星及其在种群遗传结构分析中的应用 |
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马骞1,2, 林琳1,2, 柳淑芳1, 钟声平2, 庄志猛1, 苏永全2, 唐启升1
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1.农业部海洋渔业可持续发展重点实验室， 山东省渔业资源与生态环境重点实验室
中国水产科学研究院黄海水产研究所，青岛 266071;2.厦门大学海洋与环境学院 361005
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| 摘要: |
| 利用在生长激素（GH）、生长激素释放激素（GHRH）和垂体腺苷酸环化酶激活多肽（PACAP）基因中发现的7个微卫星位点，分析了半滑舌鳎两个野生群体（渤海群体和黄海群体）和1个养殖群体间以及各群体内雌雄个体间的遗传多态性差异。结果表明，7个位点中有4个位点表现出多态性，在3个群体中的等位基因数的分布范围为2~37，平均为9.5；有效等位基因数分布范围为2~28.9，平均为8.4。各位点的平均观测杂合度、平均期望杂合度和平均多态信息含量分布范围分别为0514 5~0773 8、 0569 0~0867 1和0.482 9~0.831 4。群体间的成对FST值及个体分配分析的结果表明，半滑舌鳎野生群体和养殖群体之间存在显著性遗传差异，而在两个野生群体之间差异不显著。此外，等位基因分布和双倍体基因型分布的差异性检测结果表明，这4个多态性位点在3个群体的雌、雄性别间均不存在显著性差异。 |
| 关键词: 半滑舌鳎 生长相关基因 微卫星分析 遗传多样性 雌雄差异 |
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| 基金项目:国家自然科学基金(30871913)、山东省泰山学者工程专项(2009.03-2014.02)和山东省自主创新成果转化重大专项(2009ZHZX1A1201) |
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| Microsatellites in the growth-related genes of female and male Tongue sole Cynoglossus semilaevis and their application in the population genetic analysis |
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| Abstract: |
| Seven microsatellite loci were identified in the GH, GHRH and PACAP genes of Cynoglossus semilaevis, and were used as polymorphic markers to analyze the genetic diversity and genetic structure of two wild populations (BS and YS) and a cultured population (HS). The results revealed that no genetic polymorphism was detected in three loci out of seven. The numbers of alleles in the other four loci ranged from 2 to 37, with an average of 9.5，and the numbers of effective alleles ranged from 2 to 28.9, with an average of 8.4. The observed and expected heterozygosities ranged from 0.514 5 to 0.773 8 and from 0.569 0 to 0.867 1, respectively. The average polymorphism information content per locus ranged from 0.482 9 to 0.831 4. The results of pairwise FST and individual distribution analysis demonstrated that there was no significant difference between the wild populations, while significant differences were observed between the wild and the cultured populations. Moreover, based on the distribution of alleles and diploid genotypes, no significant difference was observed between female and male in any of the three populations. |
| Key words: Cynoglossus semilaevis Growth related gene Microsatellite analysis Genetic diversity Sexual difference |