| 摘要: |
| 随机选取池塘专养模式(M1)和稻田养殖模式(M2)养成的平均体长和体重分别为(113.37±4.55) mm、(13.35±1.23) g和(122.69±5.22) mm、(16.43±1.36) g的1龄泥鳅(Misgurnus anguillicaudatus)作为实验对象，以养殖泥鳅的稻田鲜土样为色差对照(M0)，比较分析了2种模式下泥鳅间体色、背肌质构、脏器消化酶、抗氧化酶和ATP酶的差异，以期为阐述稻田生境下泥鳅生活策略、2种养殖模式下泥鳅的品质鉴定及其养殖模式的优化提供理论依据。结果显示：(1) M1泥鳅养成品背部体色与对照具有显著差异(P<0.05)，二者测量值呈M0>M1(P<0.05)；(2) 所测背肌质构指标中，M1和M2均具显著差异(P<0.05)，除黏着性外，其余各指标测量值均呈M2>M1 (P<0.05)；(3) 所测消化酶中，M1和M2间仅蛋白酶和脂肪酶具有显著差异(P<0.05)，其测量值依次呈M2>M1和M1>M2 (P<0.05)；(4) 所测脏器抗氧化酶和消化酶中，M1和M2间均具有显著差异(P<0.05)，各测量值均呈M2>M1 (P<0.05)。 |
| 关键词: 泥鳅 养殖模式 体色 质构 消化酶 抗氧化酶 ATP酶 |
| DOI：10.11758/yykxjz.20160118001 |
| 分类号: |
| 基金项目:宁波市农业领域科技重大攻关择优委托项目(2012C10032)和浙江海洋学院优秀硕士学位论文培育项目[浙海院学位(2013)7号]共同资助 |
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| Comparison of the Body Color, Texture, Activities of Viscera Digestive Enzymes, Antioxidant Enzymes and ATP Enzymes of Two Cultured Population of Misgurnus anguillicaudatus |
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YUAN Xiangyang1, WANG Zhizheng1, YANG Cheng1, FU Yu1, LI Hongpeng1, BAI Xiaoqian1, ZHU Weidong2
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1.Fishery College, Zhejiang Ocean University, Zhoushan 316022;2.Fishery Technology Extension Center of Yuyao, Yuyao 315400
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| Abstract: |
| Misgurnus anguillicaudatus is a fish species with high economical values, and is widely distributed in China except for the Qinghai-Tibet Plateau. The popularity of this fish has been growing due to its high contents of minerals, amino acids and vitamins. Environment is one of the main factors that affect the body color, the texture and the visceral enzyme activities of the fish; hence it has been a hotspot in the research of M. anguillicaudatus. In this study we explored the living strategies of M. anguillicaudatus raised in two culture models, the pond monoculture (M1) and the polyculture of turtles, fish and shrimp in the rice field (M2). The wet earth from the paddy field served as the control (M0). We analyzed the differences in the body color, the texture, digestive enzymes, antioxidant enzymes and ATP enzymes between the two populations. The average body length and weight of the samples were (113.37±4.55) mm and (13.35±1.23) g for M1, and (122.69±5.22) mm and (16.43±1.36) g for M2. SPSS 19.0 and Excel 2003 were employed for data analysis. The results were as follows: (1) The measures of the dorsal chromatism of adult M. anguillicaudatus were M0>M1 (P<0.05). (2) There were significant differences in the measured indicators of the textures of dorsal muscles between the two populations, and all the values followed the order M2>M1 (P<0.05) except for that of adhesiveness. (3) There was no difference in the activities of the visceral digestive enzymes except for protease (M2>M1, P<0.05) and lipase (M1>M2, P<0.05). (4) Activities of antioxidant enzymes (CAT, SOD and POD) of M2 were higher than those of M1 (P<0.05). There were significant differences in activities of hepatopancreas enzymes between the two populations (P<0.05). The activities of Ca2+-Mg2+ATPase and Na+-K+ ATPase of M2 were significantly higher than those of M1 (P<0.05). |
| Key words: Misgurnus anguillicaudatus Aquaculture models Body color Texture Digestive enzymes Antioxidant enzymes ATPase |
