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| 氟苯尼考对脊尾白虾(Exopalaemon carinicauda)免疫和抗氧化功能的影响 |
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冯艳艳1,2, 李 健1,2,3, 李吉涛2, 许 杨1,2, 连春盎1,2, 翟倩倩2
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1.上海海洋大学 上海 201306;2.农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 266071;3.青岛海洋科学与技术国家实验室 海洋渔业科学与食物产出过程功能实验室 青岛 266071
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| 摘要: |
| 为研究氟苯尼考对脊尾白虾(Exopalaemon carinicauda)免疫和抗氧化功能的影响，以低、中、高(10、20和40 mg/kg·BW) 3种剂量氟苯尼考药饵连续投喂脊尾白虾5 d，对照组投喂基础饲料。于停止投喂药饵后的第3、6、12、24、48、96、168和240 h取血淋巴，测定血蓝蛋白(HEM)含量、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)和总抗氧化能力(T-AOC)等指标的变化情况。结果显示，低、中剂量组HEM含量均在3 h时间点显著高于对照组(P<0.05)，高剂量组在3–48 h显著高于对照组(P<0.05)；低、中剂量组ACP活性在3 h和6 h显著高于对照组(P<0.05)，高剂量组在3–168 h显著低于其他3组(P<0.05)；低、中剂量组AKP活性在3 h均显著高于对照组(P<0.05)，6–48 h显著低于对照组(P<0.05)，高剂量组在3–48 h显著低于对照组(P<0.05)；低、中剂量组T-SOD活性整体高于对照组，分别在6 h和24 h达到最高值(P<0.05)，高剂量组在24 h和48 h显著低于对照组(P<0.05)；低、中、高剂量组CAT活性均在3 h和6 h显著高于对照组(P<0.05)，之后在12–96 h显著低于对照组(P<0.05)，于24 h达到最低值(P<0.05)；低、中、高剂量组T-AOC活性均在3–96 h显著低于对照组(P<0.05)，于48 h达到最低值(P<0.05)，且出现剂量效应。本研究结果为氟苯尼考在海水养殖生产中使用的安全评估提供了数据支持。 |
| 关键词: 氟苯尼考 脊尾白虾 免疫 抗氧化活性 |
| DOI：10.11758/yykxjz.20160617001 |
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| Effects of Florfenicol on the Immune and Antioxidant Activities of Exopalaemon carinicauda |
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FENG Yanyan1,2, LI Jian1,2,3, LI Jitao2, XU Yang1,2, LIAN Chunang1,2, ZHAI Qianqian2
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1.Shanghai Ocean University, Shanghai 201306;2.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;3.Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071
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| Abstract: |
| The abuse of antimicrobial has raised more and more attention in recent years because of its severe harm on environments and human health caused by the drug residues. In this study, we investigated the effects of florfenicol on immune and antioxidant activities of the Exopalaemon carinicauda by measuring the concentration of HEM and the activities of ACP, AKP, T-SOD, CAT and T-AOC in the plasma of E. carinicauda in the presence of florfenicol at different concentrations. At 3 h, the HEM concentration of the samples treated with low/medium dose of florfenicol (10 or 20 mg/kg·BW) was significantly higher than that of the control group (P<0.05); from 3 h to 48 h, high dose of florfenicol (40 mg/kg·BW) had the same effect (P<0.05). At 3 h and 6 h, the ACP activity in the presence of low/medium dose of florfenicol was higher than that of the control group (P<0.05); from 3 h to 168 h, high dose of florfenicol reduced the ACP activity (P<0.05). In the presence of low/medium dose of florfenicol, the activity of AKP was higher than the control at 3 h (P<0.05), from 6 h to 48 h, however, it decreased to a lower level than the control (P<0.05). At the end of the three experiments described above, all the values of the experimental groups returned to the control level. When treated with low/medium dose of florfenicol, the activity of T-SOD was higher than the control and peaked at 6 h and 24 h; in the contrast, the T-SOD activity was lower than the control at 24 h and 48 h when treated with high dose of florfenicol (P<0.05). Regardless of the florfenicol concentration, the activity of CAT was higher than the control at 3 h and 6 h (P<0.05), but was lower than the control group from 12 h to 96 h (P<0.05), and then recovered to the control level at the end of the experiment. Florfenicol at all three concentrations caused a decrease in the T-AOC activity up to 96 h (P<0.05) in a dose-dependent manner. Our results suggested that these parameters discussed above could serve as potential biomarkers to evaluate the safe use of florfenicol in the culture of E. carinicauda. |
| Key words: Florfenicol Exopalaemon carinicauda Immune Antioxidant activity |