| 摘要: |
| 以黄海北部沿海捕获的野生黄条魳(Seriola aureovittata)为实验材料，经过体内注射植物血球凝聚素(PHA)和秋水仙素，取头肾细胞，经空气干燥法制片，Giemsa染液染色后，观察黄条魳的染色体核型和特征。本研究分析了21条黄条魳的80个中期染色体核型，结果显示，黄条魳核型有48条染色体，2n=48占所观察分裂相的比例是81.25%；第1对染色体有次缢痕和随体。核型公式为2n=48=6sm+4st+38t，其染色体臂的数量为54，单倍体染色体总长度约为51.97 μm。黄条魳染色体核型比较独特，具有3对亚中部染色体和2对亚端部染色体；不同于以往报道的其他属鱼类的染色体核型特征。通过比较分析认为，黄条为进化上的高位类群中的特化类群。本研究为属鱼类的细胞遗传学研究提供了基础资料，并为黄条魳种质资源保护及未来人工养殖等奠定基础。 |
| 关键词: 黄条魳 染色体 核型 随体 |
| DOI：10.11758/yykxjz.20160816004 |
| 分类号: |
| 基金项目:中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2016PT07)、青岛海洋科学与技术国家实验室鳌山科技创新计划专项项目(2015ASKJ02-03)和国家鲆鲽类产业技术体系(CARS-50)共同资助 |
|
| Study on the Karyotype of Yellowtail Kingfish (Seriola aureovittata) |
|
SHI Bao1,2,3,4, LIU Yongshan1,2,3,5, LIU Xuezhou1,2,3,4, XU Yongjiang1,2,3,4, LI Rong6, SONG Xuesong1,2,3,5, ZHOU Liqing1,2,3,4
|
|
1.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture;2.Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology;3.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;4.Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071;5.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;6.Dalian Fugu Fishery Co., Ltd., Dalian 116400
|
| Abstract: |
| Karyological study is a useful tool in exploring evolutionary mechanisms of fish because it provided basic information on the number, size and morphology of chromosomes. In this study we characterized the karyotype and chromosomal characteristics of the yellowtail kingfish Seriola aureovittata by examining metaphase spreads from head kidney cells of one-year-old fish artificially bred from wild broodstock in the Dalian coast of China. Chromosome samples were prepared through in vivo injection of phytohemagglutinin (PHA), olchicine-air drying technique, and Giemsa staining. A total of 80 mitotic metaphases from 21 individuals were analyzed, and 65 spreads showed 2n=48 chromosomes, which represented 81.25% of the observed metaphases. The relative size of the chromosomes in this subspecies varied from (2.50±0.23)% to (5.16±0.56)%. The long arm, short arm, and total length of the first submetacentric chromosomes were (1.81±0.09) μm, (0.87±0.03) μm, and (2.68±0.09) μm respectively. Two pairs of subtelocentric chromosomes were found in this subspecies. The length of their short arms was (0.55±0.04) μm and (0.39±0.06) μm, and that of the long arms was (2.10±0.06) μm and (1.87±0.07) μm. The diploid consisted of 4 submetacentric, 6 subtelocentric, and 38 telocentric chromosomes, and the fundamental number of chromosome arms was 54. The first pair of chromosomes had secondary constriction and satellite. The karyotype formula for this species was determined to be 2n=48 = 6sm + 4st + 38t. The total haploid chromosome length was approximately 51.97 μm. The fact that S. aureovittata has 3 pairs of submetacentric and 2 pairs of subtelocentric chromosomes distinguishes this species from other previously reported Seriola species. The comparison of karyotypes suggested that S. aureovittata might belong to an advanced and specific evolutionary group. Our study promoted the understanding of the specific karyotype evolution of the genus Seriola, and provided information on the polyploidy manipulation, hybridization, and sex control. |
| Key words: Seriola aureovittata Chromosome Karyotype Satellite |
