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| 不同温度下凡纳滨对虾和中国明对虾对白斑综合征病毒(WSSV)耐受性比较 |
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冯亚萍1,2,3, 孔 杰1,2, 罗 坤1,2, 栾 生1,2, 李旭鹏1,2, 史晓丽1,2, 曹家旺1,2,3, 王明珠1,2,3, 王 军1,2,3, 董丽君1,2,3, 孟宪红1,2
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1.农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 266071;2.青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071;3.上海海洋大学水产与生命学院 上海 201306
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| 摘要: |
| 本研究分别对凡纳滨对虾“壬海1号”(Litopenaeus vannamei “Renhai No.1”)和中国明对虾“黄海2号”(Fenneropenaeus chinensis “Huanghai No.2”)在3种温度条件下(24℃、28℃和32℃)，采用单尾定量口饲感染白斑综合征病毒(White spot syndrome virus, WSSV)的方法进行感染实验，比较2种对虾在不同温度情况下对WSSV的耐受性差异(L代表凡纳滨对虾，F代表中国明对虾)。结果显示，L-24℃和F-24℃组的平均存活时间分为(184.05±69.56) h和(101.68±38.45) h；L-28℃和F-28℃组的平均存活时间分别为(100.25±26.79) h和(73.38±22.22) h，相同温度组内均存在显著性差异(P<0.05)；截至第15天，L-32℃和F-32℃组的存活率分别为45.74%和23.47%。3个温度组对虾在50%的死亡率时的存活时间分别为178 h和98 h、98 h和74 h、292 h和78 h；死亡高峰时间分别为第5天和第4天、第5天和第4天、第10天和第4天。另外，分别在感染后的12 h、1 d、2 d、3 d、4 d、5 d、6 d、7 d、15 d共9个时间点对每组对虾进行活体取样，利用实时荧光定量RT-PCR技术对其进行病毒载量检测，从对虾体内肌肉组织病毒载量的角度探寻不同对虾抗病性能的差异。6 d时，L-24℃和F-24℃组对虾肌肉内病毒载量分别达到(2.97×106±7.44×106)和(8.08×106±3.22×106) copies/ng DNA，差异极显著(P<0.01)，L-28℃和F-28℃组分别达到(6.73×106±1.49×106)和(1.20×107±6.15×105) copies/ng DNA，差异极显著(P<0.01)；15 d，L-32℃和F-32℃组分别达到(5.18×103±4.32×103)和(3.78×104±8.97×103) copies/ng DNA，差异显著(P<0.05)。研究表明，2种对虾在3种温度环境下感染WSSV后，凡纳滨对虾耐受WSSV能力要高于中国明对虾；不同温度下同种对虾肌肉体内WSSV的增殖能力从强到弱依次为28℃组、24℃组和32℃组。 |
| 关键词: 凡纳滨对虾 中国明对虾 不同温度 白斑综合征病毒(WSSV) 耐受性 |
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| The Difference of Tolerance to White Spot Syndrome Virus Between Litopenaeus vannamei and Fenneropenaeus chinensis at Different Temperatures |
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FENG Yaping1,2,3, KONG Jie1,2, LUO Kun1,2, LUAN Sheng1,2, LI Xupeng1,2, SHI Xiaoli1,2, CAO Jiawang1,2,3, WANG Mingzhu1,2,3, WANG Jun1,2,3, DONG Lijun1,2,3, MENG Xianhong1,2
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1.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;2.Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071;3.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306
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| Abstract: |
| The present study evaluated the effects of white spot syndrome virus (WSSV) infection in Litopenaeus vannamei ‘Renhai No.1’ and Fenneropenaeus chinensis ‘Huanghai No.2’ at various temperatures (24℃, 28℃, and 32℃) using quantitative feeding method (L represents L. vannamei, and F represents F. chinensis). The results showed that the average survival time of group L-24℃, F-24℃, L-28℃, and F-28℃ was (184.05±69.56) h, (101.68±38.45) h, (100.25±26.79) h, and (73.38±22.22) h, respectively. The survival time between two kinds of shrimps at same temperature was significantly different (P<0.05). At the 15th d, the survival rate of group L-32℃ and F-32℃ was 45.74% and 23.47%, respectively. The time of 50% mortality rate of two shrimps at three temperatures was 178 h and 98 h, 98 h and 74 h, 292 h and 78 h, respectively. The time of death peak of two shrimps among three temperatures was 5 d and 4 d, 5 d and 4 d, 10 d and 4 d, respectively. The virus loads in muscle of L-24℃ and F-24℃ at 144 h was (2.97×106±7.44×106) and (8.08×106±3.22×106) copies/ng DNA with the highly significant difference (P<0.01). The virus loads in muscle of L-28℃ and F-28℃ at 144 h was (6.73×106±1.49×106) and (1.20×107±6.15×105) copies/ng DNA with highly significant difference (P<0.01). The virus loads of muscle of L-32℃ and F-32℃ at the 15th d was (5.18×103±4.32×103) and (3.78×104±8.97×103) copies/ng DNA with a significant difference (P<0.05). These results indicates that L. vannamei are more tolerant to WSSV infection than F. chinensis, and the ability of multiplication of WSSV in muscle at different temperatures was 28℃>24℃>32℃ |
| Key words: Litopenaeus vannamei Fenneropenaeus chinensis Different temperatures White spot syndrome virus (WSSV) Tolerance |
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