| 摘要: |
| Bantam能够调控细胞增殖、细胞凋亡等过程，影响生物的免疫过程。本研究利用实时荧光定量PCR(qRT-PCR)技术对感染WSSV的中国明对虾(Fenneropenaeus chinensis)肝胰腺和鳃组织内bantam表达水平进行检测，发现感染WSSV后6、12、24和48 h，中国明对虾肝胰腺中的bantam表达水平分别是对照组的(0.16±0.03)(P<0.05)、(0.63±0.26)、(0.32±0.06)(P<0.05)和(0.41±0.13)倍；中国明对虾鳃中的bantam表达水平分别是对照组的(0.30±0.17)(P<0.05)、(1.88±0.26)(P<0.01)、(0.84±0.36)和(0.51±0.25)倍。利用miRanda软件进一步对中国明对虾bantam靶基因进行预测分析，评分最高的靶基因是泛素缀合酶E2。中国明对虾泛素缀合酶E2包含UBCc功能域。多序列比对显示，UBCc功能域氨基酸残基序列在不同物种间保守性较高。进化树分析显示，分类学地位相近的物种的泛素缀合酶E2聚为一类。qRT-PCR检测感染WSSV的中国明对虾肝胰腺和鳃中的泛素缀合酶E2表达水平，结果显示，在感染WSSV后6、12、24和48 h，中国明对虾肝胰腺中泛素缀合酶E2的表达水平分别是对照组的(0.54±0.10)、(1.19±0.62)、(3.69±0.51) (P<0.01)和(1.94±0.07)(P<0.05)倍；中国明对虾鳃中泛素缀合酶E2的表达水平分别是对照组的(0.22±0.05)、(1.34±0.38)、(4.29±0.52)(P<0.01)和(1.28±0.79)倍。研究表明，bantam和泛素缀合酶E2的表达都受WSSV侵染的影响，可能与中国明对虾和WSSV之间的互作相关。但bantam和泛素缀合酶E2表达水平的变化是对虾抵抗WSSV侵染过程的免疫反应，还是宿主基因被病毒胁迫后的结果，需要进一步验证。 |
| 关键词: 中国明对虾 WSSV bantam 泛素缀合酶E2 表达 |
| DOI： |
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| Effect of White Spot Syndrome Virus Infection on bantam and Candidate Target Genes in the Chinese Shrimp Fenneropenaeus chinensis |
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LI Xupeng1, KONG Jie1,2, MENG Xianhong1, LUAN Sheng1,2, LUO Kun1
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1.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;2.Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071
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| Abstract: |
| Bantam is a type of miRNA involved in cell proliferation and apoptosis, and can affect immune processes in animals. In the present study, bantam expression in the hepatopancreas and gill of WSSV-infected Chinese shrimp Fenneropenaeus chinensis was determined using the qRT-PCR technique. The results showed that bantam expression in the hepatopancreas of Chinese shrimp 6, 12, 24, and 48 h post infection was (0.16±0.03)(P<0.05), (0.63±0.26), (0.32±0.06) (P<0.05), and (0.41±0.13) fold that of the expression in the controls, respectively. Bantam expression in the gill of Chinese shrimp 6, 12, 24, and 48 h post infection was (0.30±0.17) (P<0.05), (1.88±0.26) (P<0.01), (0.84±0.36), and (0.51±0.25) fold that of the expression in the controls, respectively. The target genes of bantam were predicted using the miRanda software. The ubiquitin-conjugating enzyme E2 was the predicted target gene with the highest score. The Chinese shrimp ubiquitin-conjugating enzyme E2 contains the ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. Multiple alignment results showed that the UBCc domain was relatively conserved among different species. Phylogenetic analysis showed that the ubiquitin-conjugating enzyme E2 in species with closer taxonomic status was clustered in a branch. Pair-wise and multiple sequence alignment revealed that the ubiquitin-conjugating enzyme E2 was relatively well-conserved. Ubiquitin- conjugating enzyme E2 expression in the hepatopancreas and gill of WSSV-infected Chinese shrimp was further determined using qRT-PCR. The results showed that the expression of ubiquitin-conjugating enzyme E2 in the hepatopancreas of Chinese shrimp 6, 12, 24, and 48 h post-WSSV infection was (0.54±0.10), (1.19±0.62), (3.69±0.51)(P<0.01), and (1.94±0.07)(P<0.05) fold that of the expression in the controls, respectively. The expression of ubiquitin-conjugating enzyme E2 in the gill of Chinese shrimp 6, 12, 24 and 48 h post WSSV infection was (0.22±0.05), (1.34±0.38), (4.29±0.52) (P<0.01), and (1.28±0.79) fold that of the expression in the controls. These results suggest that both bantam and the target gene ubiquitin-conjugating enzyme E2 might be involved in the interaction between Chinese shrimp and WSSV. Whether the altered expression of bantam and ubiquitin-conjugating enzyme E2 represents the immune response of shrimps to WSSV infection, or the hijacking of cells by WSSV during the viral infection process, needs further study. |
| Key words: Fenneropenaeus chinensis WSSV bantam Ubiquitin-conjugating enzyme E2 Expression |
