| 摘要: |
| 研究了不同温度(5℃、10℃、15℃、20℃、25℃)和光照[50、100、200 、300 μmol/(m2·s)]对铜藻(Sargassum horneri)生长、光合色素和生化组分的影响。结果显示，温度和光照对这3个方面均有显著影响(P<0.05)。铜藻在5℃～20℃、50～300 μmol/(m2·s)时均可生长，且最适生长条件为20℃、200～300 μmol/(m2·s)，特定生长率较高。温度高于25℃，藻体基本停止生长并出现腐烂现象。25℃、50 μmol/(m2·s)时，铜藻色素积累较多。可溶性蛋白和可溶性糖的含量分别在10℃、20℃时最高，不同光照间无显著性差异(P>0.05)。灰分和粗蛋白含量在5℃～10℃、50 μmol/(m2·s)时最高。粗脂肪含量在10℃ 和25℃时最低，不同光照间无显著性差异(P>0.05)。褐藻胶含量在10℃、100 μmol/(m2·s)时最高，岩藻黄素含量在10℃、50 μmol/(m2·s)时达到最大。褐藻多酚含量在低温为 5℃或高温25℃、200～300 μmol/(m2·s)高光下达到最大。研究表明，室内培养铜藻在20℃左右、200～300 μmol/(m2·s)时，生长速率最快，而铜藻在10℃、100 μmol/(m2·s)环境条件下，藻体色泽及健壮程度更好，铜藻个体大，生长速度快，可进行大规模的养殖生产。本研究结果对铜藻养殖及其活性物质提取具有重要的参考价值。 |
| 关键词: 铜藻 温度 光照强度 生长 营养组成 |
| DOI：10.19663/j.issn2095-9869.20180126001 |
| 分类号: |
| 基金项目:海洋公益性行业科研专项(201505022)和山东省现代农业产业技术体系项目(SDAIT26-01)共同资助 |
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| Effects of Culture Conditions on Nutrient Composition of Sargassum horneri |
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LUAN Qing1, LÜ Fang2, WU Haiyi2, DING Gang2, ZHAN Dongmei2
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1.College of Life Sciences, Yantai University, Yantai 264005;2.Marine Biology Institute of Shandong Province, Qingdao 266104
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| Abstract: |
| We studied the effects of temperature and light intensity on growth, photosynthetic pigment content, and nutrient components in Sargassum horneri. These factors had significant effects (P<0.05) on all three aspects. S. horneri survived at 5℃~20℃ and 50~300 μmol/(m2·s) The optimal conditions, at which specific growth rate was the highest, were 20℃ and 200~300 μmol/(m2·s). At temperatures above 25℃, the blades stopped growing, became pale, and rotted. S.horneri accumulated the most photosynthetic pigment at 25℃ and 50 μmol/(m2·s). The effects of light intensity were not significant. Soluble sugar content and soluble protein content were the highest at 10℃~20℃, and ash and crude protein content were the highest at 5℃~10℃ and 50 μmol/(m2·s). Ether extract yield was low at 10℃ and 25℃, peaked between these values, and did not vary significantly with light intensity. Alginate content was the highest at 10℃ and 100 μmol/(m2·s), and fucoxanthin content was the highest at 10℃ and 50 μmol/(m2·s). Phlorotannin content increased with increasing light intensity from 200 to 300 μmol/(m·s), where it reached a maximum; it reached local maxima at 5℃ and 25℃, and was lower between these values. S. horneri grew faster atc. 20℃ and 200~300 μmol/(m2·s), whereas color and vigor were better at 10℃ and 100 μmol/(m2·s). Its long body length and fast growth make it ideal for large-scale culturing. The nutrient components varied with culture conditions. S. horneri is a health food of moderate protein and low fat content, with high levels of functional components such as alginate, fucoxanthin, and polyphenols. It can therefore be used to provide raw material for food additives and to obtain its active components. These findings provide reference values for culturing S. horneri and extracting its components. |
| Key words: Sargassum horneri Temperature Light intensity Growth Nutrient composition |
