| 摘要: |
| 利用石蜡切片、HE和阿利新兰高碘酸雪夫试剂[AB-PAS (Alician blue and periodic acid Schiff reagent AB, pH 2.5)]染色技术，研究黄唇鱼(Bahaba flavolabiata)消化系统的组织结构和黏液细胞的分布特征。黄唇鱼食道粗而短，胃呈“卜”形，分为贲门部、盲囊部和幽门部，胃与肠的连接处有8条幽门盲囊，肠道在体腔内迂回2个曲折。光镜下观察到消化管管壁由外至内依次为外膜、肌肉层、黏膜下层和黏膜层。食道肌肉层很厚，黏膜层很薄，未见黏液细胞分布。胃部肌肉层和黏膜层均很厚，黏膜固有层分布胃腺，黏膜上皮为单层柱状上皮。幽门盲囊管腔很大，但肌肉层很薄。肠道肌肉层较厚，黏膜皱褶较细长，黏膜上皮细胞间可见圆形的黏液细胞。肝脏内弥散分布胰腺组织，肝小叶内可见大量糖原空泡。AB-PAS染色检测到胃部分布为红色的Ⅰ型黏液细胞；幽门盲囊黏膜上皮分布大量蓝色的黏液细胞，为Ⅱ型黏液细胞；前肠、中肠和后肠检测到大量Ⅱ型黏液细胞，并有少量Ⅰ型黏液细胞。黄唇鱼消化系统的组织结构以及黏液细胞分布特征体现了食性与结构功能相协调的特点。 |
| 关键词: 黄唇鱼 消化系统 黏液细胞 |
| DOI： |
| 分类号: |
| 基金项目:广州市科技计划项目(201604020088)资助 |
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| Histology and distribution of mucous cells in digestive system of Bahaba flavolabiata |
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ZHAO Yanhua1,2, OU Youjun1, LI Jiaer1, WEN Jiufu1, ZHOU Hui1,2
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1.Key Laboratory for Exploitation & Utilization of Marine Fisheries Resource in South China Sea, Ministry of Agriculture, and Rural Affairss South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300;2.National Demonstration Center for Experimental Fisheries Science Education, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306
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| Abstract: |
| This work aimed to investigate the histological structure and mucous cell distributing features in the digestive system of Bahaba flavolabiata. Conventional paraffin sections were used, with H&E and AB-PAS (Alcian blue and periodic acid Schiff reagent (AB, pH 2.5)) staining. The esophagus was crude and short, and the Bu-type stomach was divided into three portions: the cardiac stomach, fundic stomach, and pyloric stomach. There were eight pyloric caeca, and the intestine had two inflections in the body cavity. The histological analysis revealed that the digestive tract wall was composed of serosa, muscularis, submucosa, and mucosa, from the outer wall inwards. The muscularis of the esophagus was thick, but the mucosa was thin, and the epithelium did not have mucous. Muscularis and mucosa of the stomach were thick and composed of simple columnar epithelium, with a gastric gland found in the lamina propria. The pyloric caeca were characterized as having large cavity diameters, and numerous mucosa folds, with very thin muscularis layers. The muscularis in the intestine was thicker than that in the pyloric caeca, with numerous mucosa folds. Here, mucous cells were found in the epithelium. AB-PAS staining revealed that only type Ⅰ and type Ⅱ mucous cells were present in the entire digestive tract of B. flavolabiata, except in the esophagus. Numerous type Ⅰ mucous cells were found in the gastric gland and epithelium of the stomach. Only type Ⅱ mucous cells were found distributed in the epithelium of pyloric caeca mucosa. A large number of type Ⅱ and a smaller number of type Ⅰ mucous cells were found in the intestine. The former were distributed mostly on the surface of the epithelium and the latter in the basal layer of the epithelium. Characteristics of the histological structure and mucous cell distribution in digestive systems are in accordance with the habits and predacity of B. flavolabiata. |
| Key words: Bahaba flavolabiata Digestive system Mucous cell |
