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| 金乌贼端粒酶逆转录酶基因鉴定和组织差异表达分析 |
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项子龙1,2,3, 刘长琳2, 张金勇2,3,4, 胡鹏2,3, 何暮春5, 柳淑芳2,3, 庄志猛2, 薛同明6
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1.上海海洋大学水产与生命学院 上海 201306;2.中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 青岛 266071;3.青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071;4.南京农业大学无锡渔业学院 无锡 214081;5.中国海洋大学生命学院 青岛 266003;6.青岛金沙滩水产开发有限公司 青岛 266000
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| 摘要: |
| 本研究从金乌贼(Sepia esculenta)转录组中筛选出疑似TERT (Telomerase reverse transcriptase)基因序列，通过系统进化和同源序列分析，鉴定出该基因为金乌贼TERT基因，序列长度为2415 bp。采用实时荧光定量PCR技术，检测和分析金乌贼TERT基因在不同组织、不同发育时期和不同性别的相对表达量差异。结果显示，与产卵期相比，濒死期的心、肝、胰、鳃、性腺、食道上神经团(除去视腺和视叶后的脑组织)等6个组织的TERT基因表达量呈显著下调趋势，推测与濒死期的器官组织分裂和增殖能力下降有关。TERT基因在金乌贼不同组织中具有明显的组织特异性，说明金乌贼各组织器官的衰老状态并不一致。本研究结果可为深入研究头足类生殖与衰老等生命过程的端粒酶调控机制提供基础数据。 |
| 关键词: 金乌贼 TERT基因 qRT-PCR 系统进化树 基因差异表达分析 |
| DOI：10.19663/j.issn2095-9869.20190326001 |
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| Gene Identification and Tissue Differential Expression Analysis of Sepia esculenta Telomerase Reverse Transcriptase |
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XIANG Zilong1,2,3, LIU Changlin2, ZHANG Jinyong2,3,4, HU Peng2,3, HE Muchun5, LIU Shufang2,3, ZHUANG Zhimeng2, XUE Tongming6
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1.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071;3.Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071;4.Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081;5.College of Marine Life Sciences, Ocean University of China, Qingdao 266003;6.Qingdao Jinshatan Aquatic Product Development Co., Ltd., Qingdao 266000
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| Abstract: |
| Telomerase reverse transcriptase (TERT) gene is a key structure and major regulatory subunit of telomerase. TERT synthesizes telomeric DNA repeats by reverse transcription of telomerase RNA template sequences and adds them to the ends of chromosomes to maintain and extend the ends. Golden cuttlefish (Sepia esculenta) is an annual species that dies quickly after spawning. This characteristic makes it a potential research object for the study of reproduction and aging. In this study, the phylogenetic and homologous sequence analysis revealed that the gene was a Sepia esculenta TERT gene with a sequence length of 2415 bp. Quantitative Real-time PCR (qRT-PCR) was used to detect the relative expression of TERT gene in the growth stages, spawning stages and aging stages of S. esculenta, and to analyze life cycle differences and gender differences. The research results show that TERT gene expression in six tissues, including heart, liver, pancreas, gill, gonad and esophageal nerve mass, showed a downward trend, which was supposed to be related to the decline of organ division and proliferation in the near-death stage. TERT gene has obvious specificity in different tissues of, Sepia esculenta. It shows that the aging status of different tissues and organs of Sepia esculenta is different. The results of this study can provide basic data for the further study of the regulation mechanism of Telomerase in reproduction and senescence of cephalopod. |
| Key words: Sepia esculenta TERT gene qRT-PCR Phylogenetic tree Gene differential expression analysis |