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| 基于微卫星分子标记的凡纳滨对虾商业苗种遗传多样性分析 |
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方振朋1,2, 孟宪红2, 李旭鹏2, 栾生2, 曹家旺2, 陈宝龙2, 孔杰2, 闫茂仓3, 胡利华3
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1.上海海洋大学水产与生命学院 上海 201306;2.中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071;3.浙江省近岸水域生物资源开发与保护重点实验室 温州 325005
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| 摘要: |
| 为研究我国凡纳滨对虾(Litopenaeus vannamei)商业苗种的遗传多样性特征，于河北、山东、广东、海南等地采集了6个有代表性的凡纳滨对虾品牌苗种，分别命名为黄骅R、东营M、广州P、广州Z、海南S和海南Z，以8个微卫星标记检测其遗传多样性。结果显示，6个品牌的凡纳滨对虾在8个位点呈现不同程度的多态性，其平均等位基因数(Na)、期望杂合度(He)、观测杂合度(Ho)和多态信息含量(PIC)分别为4.5~9.5、0.516~0.733、0.346~0.550和0.472~0.700，各品牌遗传多样性丰富程度从高到低分别为黄骅R>广州Z>广州P>海南Z>东营M>海南S。哈迪–温伯格平衡(HWE)检验显示，4.17% (2/48)的检测结果表现为显著的偏离(0.01
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| 关键词: 凡纳滨对虾 商业苗种 微卫星 遗传多样性 |
| DOI：10.19663/j.issn2095-9869.20190527004 |
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| Genetic Diversity Analysis of Domestic Commercial Brands Seedlings of Litopenaeus vannamei Based on Microsatellite Molecular Markers |
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FANG Zhenpeng1,2, MENG Xianhong2, LI Xupeng2, LUAN Sheng2, CAO Jiawang2, CHEN Baolong2, KONG Jie2, YAN Maocang3, HU Lihua3
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1.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Laboratory of Marine Fisheries Science and Food Production Processes, Pilot National Laboratory of Marine Science and Technology (Qingdao), Qingdao 266071;3.Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource, Wenzhou 325005
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| Abstract: |
| To clarify the genetic diversity characteristics of domestic commercial brand seedlings of Litopenaeus vannamei, six domestic commercial brands of L. vannamei, namely, Huanghua R, Dongying M, Guangzhou P, Guangzhou Z, Hainan S, and Hainan Z, were collected from Hebei, Shandong, Guangdong, and Hainan Province. Their genetic diversity was detected using eight microsatellite markers. The results showed that the average allele number (Na), expected heterozygosity (He), observed heterozygosity (Ho), and polymorphic information content (PIC) were 4.5~9.5, 0.516~0.733, 0.346~0.550, and 0.472~0.700, respectively. The genetic diversity of the six brands ranged from high to low: Huanghua R > Guangzhou Z > Guangzhou P > Hainan Z >Dongying M > Hainan S, and 48 Hardy-Weinberg equilibrium tests were conducted on the 6 brands of L. vannamei using 8 microsatellite loci. It was observed that, 4.17% (2/48) showed significant deviation (0.01
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| Key words: Pacific white shrimp Litopenaeus vannamei Commercial seedling Microsatellite Genetic diversity |