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| 真蛸染色体核型分析 |
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李凤辉1,2, 边力2,3, 史会来4, 周丽青2, 陈四清2,3, 葛建龙2,3, 刘长琳2,3, 卢斌4, 常青2,3
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1.上海海洋大学水产科学国家级实验教学示范中心 水产动物遗传育种中心上海市协同创新中心 上海水产养殖工程技术研究中心 上海 201306;2.中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 山东 青岛 266071;3.青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 山东 青岛 266071;4.浙江省海洋水产研究所 浙江省海水增养殖重点实验室 浙江 舟山 316021
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| 摘要: |
| 以东海沿海捕获的野生真蛸(Octopus sinensis)为实验材料，用含秋水仙素终浓度为0.005%的海水活体暂养的方法，取鳃和肾脏细胞，利用热滴片法制备染色体标本，对其核型进行分析。结果显示，真蛸的二倍体染色体条数为60条，核型公式为2n=14 m+26 sm+12 st+8 t，其染色体臂数(NF)为100。未发现带有随体和次溢痕的染色体和异型染色体，推断真蛸可能为常染色体性别决定类型。本研究可为蛸属的细胞遗传学研究及真蛸种质鉴定和资源保护提供基础资料和参考依据。 |
| 关键词: 真蛸 染色体 核型 |
| DOI：10.19663/j.issn2095-9869.20200324001 |
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| Karyotype analysis of the East Asian common octopus, Octopus sinensis |
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LI Fenghui1,2, BIAN Li2,3, SHI Huilai4, ZHOU Liqing2, CHEN Siqing2,3, GE Jianlong2,3, LIU Changlin2,3, LU Bin4, CHANG Qing2,3
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1.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai Engineering Research Center of Aquaculture, Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai 201306, China;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao, Shandong 266071, China;3.Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, Shandong 266237, China;4.Zhejiang Province Key Laboratory of Mariculture and Enhancement, Marine Fisheries Research Institute of Zhejiang, Zhoushan, Zhejiang 316021, China
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| Abstract: |
| Comparative studies of the karyotypes of closely related species may aid our understanding of the mechanisms of chromosomal evolution relevant to speciation. It may also help to clarify phylogenetic relationships. In the present study, we analyzed the karyotypes of ten wild immature East Asian common octopus (Octopus sinensis; 5 male and 5 female) from the coastal regions of the East China Sea. O. sinensis were held in seawater containing 0.005% colchicine for 2 h, and then the gill and kidney were collected for chromosome sample preparation via the hot-dropping method. The results showed that the diploid chromosomal number of O. sinensis is 60, and the karyotype formula is 2n = 14m + 26sm + 12st + 8t, NF = 100. No satellite, secondary constriction, or heteromorphic chromosomes of sex were observed. Therefore, we speculate that the sex determination mechanism of O. sinensis is autosomal. This study provides basic data for cytogenetic studies of Octopus and serves as a valuable reference for O. sinensis germplasm identification and conservation. |
| Key words: Octopus sinensis Chromosome Karyotype |