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| 棕点石斑鱼(♀)与蓝身大斑石斑鱼(♂)及其杂交子代遗传多样性分析 |
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吴玉萍1,2, 田永胜1,3, 王林娜1,3, 李振通1,4, 张晶晶1,4, 黎琳琳1,5, 李子奇1,4, 陈帅1,4, 马文辉6, 王清滨6, 李波6
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1.中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 山东 青岛 266071;2.大连海洋大学水产与生命学院 辽宁 大连 116023;3.青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 山东 青岛 266071;4.上海海洋大学水产与生命学院 上海 201306;5.中国农业科学院 北京 100081;6.莱州明波水产有限公司 山东 莱州 261400
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| 摘要: |
| 本研究利用20对石斑鱼微卫星引物对金虎斑[棕点石斑鱼(Epinephelus fuscoguttatus) (♀)´蓝身大斑石斑鱼(E. tukula) (♂)]及其父母本进行微卫星分析，从分子水平对金虎斑及其双亲的遗传多样性进行研究。结果显示，20个微卫星位点共检测到215个等位基因，平均多态信息含量(PIC)为0.638 6；棕点石斑鱼平均有效等位基因数(Ne)最多(4.367 9)，金虎斑次之(3.370 6)，蓝身大斑石斑鱼最少(2.412 8)；蓝身大斑石斑鱼、棕点石斑鱼和金虎斑3个群体平均观测杂合度(Ho)分别为0.385 5、0.474 9和0.473 6，PIC分别为0.491 9、0.645 3和0.555 1；3种石斑鱼群体群内近交系数(Fis)、总群体近交系数(Fit)、遗传分化系数(Fst)和基因流(Nm)平均值分别为0.228 0、0.344 1、0.150 4和1.411 9，群体间分化较大；采用UPGMA法构建的聚类分析图显示，金虎斑与棕点石斑鱼先聚为一类，二者亲缘关系更近。研究表明，金虎斑存在较丰富的遗传多样性表现，在遗传学方面可进行杂交石斑鱼进一步选育及杂种优势研究。 |
| 关键词: 棕点石斑鱼 蓝身大斑石斑鱼 杂交 微卫星标记 遗传多样性 |
| DOI：10.19663/j.issn2095-9869.20200707001 |
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| Genetic diversity analysis of Epinephelus fuscoguttatus (♀) and E. tukula (♂) hybrids |
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WU Yuping1,2, TIAN Yongsheng1,3, WANG Linna1,3, LI Zhentong1,4, ZHANG Jingjing1,4, LI Linlin1,5, LI Ziqi1,4, CHEN Shuai1,4, MA Wenhui6, WANG Qingbin6, LI Bo6
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1.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China;2.College of Fisheries and Life Science, Dalian Ocean University, Dalian, Liaoning 116023, China;3.Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, Shandong 266071, China;4.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;5.Chinese Academy of Agricultural Sciences, Beijing 100081, China;6.Mingbo Aquatic Co. Ltd., Laizhou, Shandong 261400, China
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| Abstract: |
| The genetic diversity of Jinhu grouper hybrids (Epinephelus fuscoguttatus ♀ ×E. tukula ♂) and their parents were analyzed via microsatellites. The results detected 215 alleles at 20 microsatellite loci, with an average polymorphic information content (PIC) of 0.638 6. The highest average effective allele number (Ne) was measured in E. fuscoguttatus (4.367 9), followed by the hybrids (3.370 6) and E. tukula (2.412 8). The average observed heterozygosity values (Ho) were 0.385 5 (E. tukula), 0.474 9 (E. fuscoguttatus), and 0.473 6 (hybrids), and the average PIC values were 0.491 9, 0.645 3, and 0.555 1, respectively. The three grouper populations were averaged to calculate the inbreeding coefficient (Fis; 0.228 0), total population inbreeding coefficient (Fit; 0.344 1), genetic differentiation coefficient (Fst; 0.150 4), and gene flow (Nm; 1.411 9), but differentiation among the populations was also observed. Cluster analysis, performed by the unweighted pair group method with arithmetic mean, placed the hybrids and E. fuscoguttatus into one group, indicating a close relationship. Prior studies showed that the Jinhu grouper is genetically diverse, which allowed for selective breeding, hybrid generation, and exploration of the genetic basis of heterosis. |
| Key words: Epinephelus fuscoguttatus E. tukula Hybrid Microsatellite markers Genetic diversity |
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