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耳石锶标记识别鮻放流群体的可行性研究
王硕1,2, 张博伦1,2, 郭彪1,2, 姜涛3, 陈卫1,2, 杨健3, 高燕1,2, 曾祥茜1,2
1.天津市水产研究所 天津 300457;2.天津市海洋牧场技术工程中心 天津 300457;3.中国水产科学研究院淡水渔业研究中心 江苏 无锡 214081
摘要:
为探究耳石锶(Sr2+)标记技术在鮻(Liza haematocheila)增殖放流群体识别的可行性,以体长5 cm左右的鮻幼鱼为研究对象,将其养殖海水中Sr2+的质量浓度分别调至50、100、200和400 mg/L,以自然海水(Sr2+质量浓度为7.9 mg/L)为对照组,人为改变鮻幼鱼生存环境中Sr2+的质量浓度标记48 h。同时开展双环标记实验(在鮻3 cm幼鱼时进行Sr2+质量浓度200 mg/L海水96 h标记,待其长至5 cm幼鱼时进行Sr2+质量浓度100 mg/L海水48 h标记)。实验结果显示,Sr2+质量浓度50~400 mg/L的海水48 h标记的鮻耳石Sr/Ca比均出现一个显著的上升阶段且面分析出现明显的“高Sr2+标记环”。综合本研究结果和标记效率,推荐采用Sr2+质量浓度为50 mg/L的海水进行鮻耳石Sr2+标记。双环标记实验鮻耳石Sr2+元素面分布结果出现层次分明的双环,且双环标记组鮻耳石2条不同标记环的线性分析结果与其相对应的单环标记组基本一致,这表明通过Sr2+标记次数来进行不同放流群体识别是可行的。
关键词:    耳石  Sr2+标记  Sr2+质量浓度  双环标记
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Study of the Feasibility of Identifying the Group of Released Liza haematocheila by Using the Strontium Marking Method in Otoliths
WANG Shuo1,2, ZHANG Bolun1,2, GUO Biao1,2, JIANG Tao3, CHEN Wei1,2, YANG Jian3, GAO Yan1,2, ZENG Xiangxi1,2
1.Tianjin Sea Fisheries Research Institute, Tianjin 300457, China;2.Center for Marine Ranching Engineering Science Research of Tianjin, Tianjin 300457, China;3.China Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu 214081, China
Abstract:
To study the feasibility of using the strontium marking method in otoliths to identify the group of released Liza haematocheila, juveniles with a total length of ~5 cm were immersed in four different concentrations (50, 100, 200, and 400 mg/L) of Sr2+ for 48 h to detect the Sr2+ sedimentation effect. A double ring marking experiment was also conducted: L. haematocheila juveniles with a total length of about 3 cm were immersed in seawater with Sr2+concentrations of 200 mg/L, for 96 h. When the length of the first marked juveniles reached 5 cm, half of them were again immersed in seawater with Sr2+ concentrations of 100 mg/L, for 48 h. A significant peak was observed in EPMA (electron probe microanalysis) line transect analysis and a "high strontium marking ring" appeared in EPMA mapping analysis of L. haematocheila otoliths in all the marked treatments. Based on the results of the experiment and the marking efficiency, we suggest that a marking concentration of 50 mg/L Sr2+ could be used for strontium marking on otoliths of L. haematocheila. A well-structured double marking ring was observed in EPMA mapping analysis of L. haematocheila otoliths in double ring marking treatment, and the EPMA line transect analysis result of each ring in double-ring marking juveniles otoliths was the same as that in the corresponding monocyclic ring treatment. These results suggest that it is feasible to identify different groups of released L. haematocheila by changing the strontium marking times.
Key words:  Liza haematocheila  Otolith  Strontium marking  Concentration of Sr2+  Double ring mark