不同氮营养盐对赫氏颗石藻和三角褐指藻中DMSP含量的影响

李凌霄; 孙婧; 宋若晗; 崔正国; 曲克明; 王庆奎; 周明莹; 崔鸿武; 胡清静

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不同氮营养盐对赫氏颗石藻和三角褐指藻中DMSP含量的影响
李凌霄^1,2, 孙婧³, 宋若晗^1,2, 崔正国², 曲克明⁴, 王庆奎¹, 周明莹², 崔鸿武⁴, 胡清静⁵
1.天津农学院水产学院天津 300392;2.中国水产科学研究院黄海水产研究所海水养殖生物育种与可持续产出全国重点实验室农业农村部海洋渔业与可持续发展重点实验室山东青岛 266071;3.山东省海洋资源与环境研究院山东省海洋生态修复重点实验室山东烟台 264006;4.中国水产科学研究院黄海水产研究所海水养殖生物育种与可持续产出全国重点实验室农业农村部海洋渔业与可持续发展重点实验室山东青岛 266072;5.中国水产科学研究院黄海水产研究所海水养殖生物育种与可持续产出全国重点实验室农业农村部海洋渔业与可持续发展重点实验室山东青岛 266073

摘要:

二甲基巯基丙酸内盐(dimethylsulfoniopropionate, DMSP)是地球上重要的有机硫化合物之一，在全球硫循环和气候调节等方面发挥着重要作用。浮游植物是海洋环境中DMSP的主要生产者，而氮营养盐是影响浮游植物产生DMSP的重要因素之一。本研究以赫氏颗石藻(Emiliania huxleyi) (DMSP高产量藻)与三角褐指藻(Phaeodactylum tricornutum)(DMSP中等产量藻)为对象，通过室内培养实验，对比分析了在不同氮营养盐浓度及不同氮营养盐类型条件下，2种藻培养液中颗粒态DMSP (DMSPp)的含量及其与藻密度、单细胞DMSPp含量的关系。研究发现，不同氮营养盐浓度或氮营养盐类型对赫氏颗石藻单细胞DMSPp含量影响较小(P>0.05)，培养液中的DMSPp浓度主要受藻细胞密度影响；而不同氮营养盐浓度或氮营养盐类型对三角褐指藻单细胞的DMSPp含量影响显著(P<0.05)，培养液中的DMSPp浓度主要受藻的单细胞DMSPp含量影响。例如，对于三角褐指藻来说，低NO3–浓度(0 μmol/L)培养组单细胞DMSPp平均含量是高NO3–浓度(1 764 μmol/L)培养组的11倍；在不同氮营养盐类型下，NaNO3培养组中总DMSPp的平均浓度分别为NH4Cl和CH4N2O培养组的3~4倍。推测以上差异可能与DMSP在不同藻类中的生理作用存在差异有关。

关键词: 二甲基巯基丙酸内盐(DMSP) 赫氏颗石藻三角褐指藻氮营养盐

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Effect of different nitrogen nutrients on DMSP content in Emiliania huxleyi and Phaeodactylum tricornutum

LI Lingxiao^1,2, SUN Jing³, SONG Ruohan^1,2, CUI Zhengguo⁴, QU Keming⁴, WANG Qingkui⁵, ZHOU Mingying⁴, CUI Hongwu⁴, HU Qingjing⁴

1.College of Fishery, Tianjin Agricultural University, Tianjin 300392, China;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Key Laboratory of Marine Fisheries and Sustainable Development, Ministry of Agriculture and Rural Affairs, Qingdao 266071, Chin;3.Shandong Marine Resource and Environment Research Institute, Shandong Key Laboratory of Marine Ecological Restoration, Yantai 264006, China;4.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Key Laboratory of Marine Fisheries and Sustainable Development, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China;5.College of Fishery, Tianjin Agricultural University, Tianjin 300392

Abstract:

Dimethylsulfoniopropionate (DMSP) is one of the most important organic sulfur compounds on Earth, and its significance in global sulfur cycling and climate regulation cannot be overlooked, as it plays an indispensable role in these processes. Phytoplankton are the major producers of DMSP in the marine environment, and nitrogen nutrients are key factors influencing the production of DMSP in phytoplankton. This study focused on two algal species, Emiliania huxleyi (a high DMSP producer) and Phaeodactylum tricornutum (a medium DMSP producer), and conducted indoor culture experiments to compare and analyze the content of particulate DMSP (DMSPp) in the algal culture media under different nitrogen nutrient concentrations and types. The study investigated the relationships between overall DMSPp content, algal density, and DMSPp content per individual algal cell. The results indicated that different nitrogen nutrient concentrations and types had a minimal impact on the content of DMSPp per individual cell in E. huxleyi (P>0.05), suggesting that the DMSPp concentration in the culture media was mostly influenced by algal cell density. Conversely, different nitrogen nutrient concentrations and types had a significant impact on the content of DMSPp per individual cell in P. tricornutum (P<0.05), indicating that the DMSPp concentration in the culture media was mainly influenced by the content of DMSPp per individual algal cell. For instance, in the case of P. tricornutum, the average DMSPp content per individual cell in the low NO3– concentration (0 μmol/L) culture group was 11 times greater than that in the high NO3– concentration (1 764 μmol/L) culture group. Furthermore, under different nitrogen nutrient types, the average total DMSPp concentration in NaNO3 culture media was three and four times higher than that in the NH4Cl and CH4N2O culture groups, respectively. These differences may be attributed to variations in the physiological effects of DMSP on different algal species.

Key words: Dimethylsulfoniopropionate Emiliania huxleyi Phaeodactylum tricornutum Nitrogen nutrient